logo-web-IIThe laboratory collaborates with academic institutions, pharmaceutical companies, analytical platforms and scientific software developers. Its main research topics pertain to the field of clinical applications of targeted and untargeted metabolomics of plasma, cells, tissues and urine, to therapeutic drug monitoring and drug metabolisation studies, and to the analysis of small molecules by the use of profiling separation methods coupled with mass spectrometry detection.

 

  • Plasma metabolic profiling of asthmatic and cystic fibrosis mice models – Prof. D Radzioch, Department of Human Genetics, McGill University, Montreal, Quebec, Canada
  • Statistical tools development – Prof. P Filzmoser, Department of Statistics and Probability Theory, Vienna University of Technology, Vienna, Austria
  • Prediction of orthogonality for comprehensive two-dimensional separations by second dimension retention indices – Prof. J-M Dimandja, Spelman College, Atlanta, Georgia, USA
  • Metabolite profiling of serum and urine from horses with atypical myopathy – MVDr. Petr Jahn, CSc., Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
  • Optimisation column combination for orthogonal GC×GC-TOF MS based clinical metabolomics – Dr. M. Merrick, LECO Instrumente and Prof. Jack Cochran, Restek
  • Mass spectrometry tools development for untargeted metabolomics – Thermo Fisher Scientific, San Jose, Silicon Valley, CA, USA
  • Defining disease mechanisms and treatment response of chronic myeloid leukaemia by metabolomics; High throughput method for therapeutic drug monitoring and drug metabolisation study – Prof. Edgar Faber, Department of Hemato-Oncology, University Hospital Olomouc, Faculty of Medicine and Dentistry, Palacky University, Czech Republic
  • Improving technologies for metabolomics and software robots decision algorithms – Dr R Mistrik, HighChem Ltd., Bratislava, Slovakia
  • Identification new biomarkers by multistage fragmentation spectra – Prof. Ron A Vewers, Translational Metabolic Laboratory, Radboud University Medical Centre, Nijmegen, the Netherlands
  • Metabolomics of tauopaties – Dr. Andrej Kovac, Institute of Neuroimmunology, Slovak Academy of Sciences, Bratislava, Slovak Republic
  • Breast Cancer tissue metabolomics, Prof. Michal Holčapek, Department of Analytical Chemistry Pardubice, Faculty of Chemical Technology, University of Pardubice, Czech Republic

 

 

 


Papers (2010-2016)


  • Vrobel I, Janečková H, Faber E, Bouchalová K, Mičová K, Friedecký D, Adam T. Ultrafast Online SPE-MS/MS Method for Quantification of 3 Tyrosine Kinase Inhibitors in Human Plasma. Ther Drug Monit. 2016 Aug;38(4):516-24.
  • Gardlo A, Smilde A, Hron K, Hrdá M, Karlíková R, Friedecký D, Adam T. Normalization techniques for PARAFAC modeling of urine metabolomics data. Metabolomics 2016
  • Templ M, Hron K, Filzmoser P, Gardlo A. Imputation of rounded zeros for high-dimensional compositional data. Chemometrics and Intelligent Laboratory Systems 2016;155:183-190.
  • Karlíková R, Širková J, Jahn P, Friedecký D, Gardlo A, Janečková H, Hrdinová F, Drábková Z, Adam T. Equine atypical myopathy: A metabolic study. The Veterinary Journal 2016,216:125-132.
  • Karlíková R, Široká J, Friedecký D, Faber E, Hrdá M, Mičová K, Fikarová I, Gardlo A, Janečková H, Vrobel I, Adam T. Metabolite Profiling of the Plasma and Leukocytes of Chronic Myeloid Leukemia Patients. J Proteome Res. 2016.
  • Ligasová A, Liboska R, Friedecký D, Mičová K, Adam T, Oždian T, Rosenberg I and Koberna K. Dr Jekyll and Mr Hyde: A Strange Case of 5-Ethynyl-2′-deoxyuridine and 5-ethynyl-2′-deoxycytidine. Open Biol. 2016 Jan;6(1):150172.
  • Zezulová M, Bartoušková M, Hlídková E, Juráňová J, Červinková B, Kasalová E, Adam T, Krčmová LK, Solichová D, Cwiertka K, Vrána D, Melichar B. Prognostic Significance of Serum and Urinary Neopterin Concentrations in Patients with Rectal Carcinoma Treated with Chemoradiation. Anticancer Res. 2016 Jan;36(1):287-92.
  • Zezulová M, Bartoušková M, Hlídková E, Adam T, Krčmová LK, Červinková B, Solichová D, Zlevorová M, Cwiertka K, Friedecký D, Vrána D, Melichar B. Citrulline as a biomarker of gastrointestinal toxicity in patients with rectal carcinoma treated with chemoradiation. Clin Chem Lab Med. 2016 Feb;54(2):305-14.
  • Najdekr L, Gardlo A, Mádrová L, Friedecký D, Janečková H, Correa ES, Goodacre R, Adam T. Oxidized phosphatidylcholines suggest oxidative stress in patients with medium-chain acyl-CoA dehydrogenase deficiency. Talanta. 2015;139:62-6.
  • Faber E, Divoká M, Skoumalová I, Novák M, Marešová I, Mičová K, Friedecký D, Adam T, Jarošová M, Indrák K. A lower dosage of imatinib is sufficient to maintain undetectable disease in patients with chronic myeloid leukemia with long-term low-grade toxicity of the treatment. Leuk Lymphoma. 2015 22:1-6.
  • Friedecký D, Mičová K, Faber E, Hrdá M, Široká J, Adam T. Detailed study of imatinib metabolization using high-resolution mass spectrometry. J Chromatogr A. 2015;1409:173-81.
  • Ligasová A, Strunin D, Friedecký D, Adam T, Koberna K. A fatal combination: a thymidylate synthase inhibitor with DNA damaging activity. PLoS One. 2015;10(2):e0117459.
  • Kalivodová A, Hron K, Filzmoser P, Najdekr L, Janečková H and Adam T. PLS-DA for compositional data with application to metabolomics. Journal of Chemometrics 2015 29:21-28.
  • Janeckova H, Kalivodova A, Najdekr L, Friedecky D, Hron K, Bruheim P, Adam T. Untargeted metabolomic analysis of urine samples in the diagnosis of some inherited metabolic disorders. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015 Dec;159(4):582-5.
  • Veleba J., Kopecky J., Janovska P., Kuda O., Horakova O., Malinska H., Kazdova L., Oliyarnyk O., Skop V., Trnovska J., Hajek M., Skoch A., Flachs P., Bardova K., Rossmeisl M., Olza J., Salim de Castro G., Calder P.C., Gardlo A., Fiserova E., Jensen J., Bryhn M., Kopecky J., Pelikanova T., Combined intervention with pioglitazone and n-3 fatty acids in metformin-treated type 2 diabetic patients: improvement of lipid metabolism. Nutr Metab. 2015 2;12:52.
  • Študentová H, Vitásková D, Šrámek V, Indráková J, Adam T, Juráňová J, Petrová P, Kujovská Krčmová L, Pešková E, Solichová D, Kalábová H, Melichar B. Correlations of neutrophil-to-lymphocyte, lymphocyte-to-monocyte and platelet-to-lymphocyte ratios with biomarkers of atherosclerosis risk and inflammatory response in patients with a history of breast cancer. Pteridines 2015;26(4):161-172.
  • KANAGARATHAM, C., A. GARDLO, L. NAJDEKR, D. FRIEDECKÝ, T. ADAM, M. HAJDÚCH, J.B. DE SANCTIS a D. RADZIOCH. Fenretinide prevents inflammation and airway hyperresponsiveness in a mouse model of allergic asthma. American Journal of Respiratory Cell and Molecular Biology. 2014, ISSN 1044-1549. IF (2013): 4.109. PMID: 24885263
  • Kalivodová A., Hron K., Filzmoser P. Najdekr L. Janečková H. and Adam T. PLS-DA for compositional data with application to metabolomics. Journal of Chemometrics, 2014, doi: 10.1002/cem.2657
  • Wojtowicz, Petr; Janeckova, Hana; Friedecky, David; et al. Metabolomic Techniques in Biomedicine. Chem Papers. 2013; 107(1):3-11.
  • Rohon P, Faber E, Divoka M, Rozmanova S, Friedecky D, Jarosova M, Indrak K. A significant proportion of patients with chronic myeloid leukemia and suboptimal response according to European Leukemia Net criteria have excellent prognosis without treatment change. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2013 Jun;157(2):181-8.
  • Cizkova M, Bouchalova K, Friedecky D, Polynkova A, Janostakova A, Radova L, Cwiertka K, Trojanec R, Zezulova M, Zlevorova M, Hajduch M, Melichar B. High lapatinib plasma levels in breast cancer patients: risk or benefit? Tumori. 2012 Jan-Feb;98(1):162-5.
  • Micová K, Friedecký D, Faber E, Adam T. Isotope dilution direct injection mass spectrometry method for determination of four tyrosine kinase inhibitors in human plasma. Talanta. 93 (2012) 307-13.
  • Béres T, Gemrotová M, Tarkowski P, Ganzera M, Maier V, Friedecký D, Dessoy MA, Wessjohann LA, Spíchal L, Strnad M, Doležal K. Analysis of cytokinin nucleotides by capillary zone electrophoresis with diode array and mass spectrometric detection in a recombinant enzyme in vitro reaction. Anal Chim Acta. 2012 Nov 2;751:176-81.
  • Faber E, Friedecký D, Micová K, Rožmanová S, Divoká M, Jarošová M, Indrák K, Adam T. Imatinib trough plasma levels do not correlate with the response to therapy in patients with chronic myeloid leukemia in routine clinical setting. Ann Hematol. 91 (2012) 923-9. IF(2010) = 2.600
  • Isotope dilution direct injection mass spectrometry method for determination of four tyrosine kinase inhibitors in human plasma. Mičová K, Friedecký D, Faber E, Adam T. Talanta. 2012 May 15;93:307-13.
  • Janecková H, Hron K, Wojtowicz P, Hlídková E, Barešová A, Friedecký D, Žídková L, Hornik P, Behúlová D, Procházková D, Vinohradská H, Pešková K, Bruheim P, Smolka V, Stastná S, Adam T. Targeted metabolomic analysis of plasma samples for the diagnosis of inherited metabolic disorders. J Chromatogr A. 1226 (2012) 11-7.
  • Zrostlikova J, Binkley J, Wojtowicz P, Adam T. Application of comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry in human metabolomic studies. J Anal Chem 66 (2011) 1333-1340.
  • Bruheim, P., Kvitvang, H. F. N., Andreassen, T., Adam, T. & Villas-Boas, S. G. Highly Sensitive GC/MS/MS Method for Quantitation of Amino and Nonamino Organic Acids. Anal Chem 83, 2705-2711.
  • Bouchalová K, Cížková M, Cwiertka K, Trojanec R, Friedecký D, Hajduch M. Lapatinib in breast cancer – the predictive significance of her1 (egfr), her2, pten and pik3ca genes and lapatinib plasma level assessment. Biomed Pap Med Fac Univ Palacky Olomouc. 154 (2010) 281-288.
  • Friedecky, D., Micova, K., Faber, E., Polynkova, A. & Adam, T. Flow injection analysis vs. ultra high performance liquid chromatography coupled with tandem mass spectrometry for determination of imatinib in human plasma. Clin Chim Acta 411, 1957-1962.
  • Faber, E. et al. Imatinib dose escalation in two patients with chronic myeloid leukemia, with low trough imatinib plasma levels measured at various intervals from the beginning of therapy and with suboptimal treatment response, leads to the achievement of higher plasma levels and major molecular response. Int J Hematol 91, 897-902.
  • Zidkova, L. et al. Oligodendroglia from ADSL-deficient patient produce SAICAribotide and SAMP. Mol Genet Metab 101, 286-288.
  • Adam, T., Wojtowicz, P., Zrostlikova, J., Kovalczuk, T. & Schurek, J. Evaluation of comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry for the diagnosis of inherited metabolic disorders using an automated data processing strategy. J Chromatogr A 1217, 8054-8061.

 


The most important past projects of the laboratory


  • Biomedicine for regional development and human resources. In collaboration with the Institute of Organic Chemistry and Biochemistry AS CR, v.v.i., Prague, Czech Republic; and the Institute of Chemical Technology, Prague, Czech Republic
  • Diagnostic tools of inherited diseases of purine and pyrimidine metabolism. In collaboration with Dr A H Simmonds (Purine Research Laboratory, Guy’s Hospital, London, UK);
  • Development of treatment of Diamond-Blackfan anemia patients. In collaboration with Professor D Pospisilova (Department of Paediatrics, University Hospital Olomouc, Faculty of Medicine and Dentistry, Palacky University, Czech Republic);
  • Personalised treatment of chronic myeloproliferative disorders and myelodysplastic syndrome – a cellular metabolomics study. In collaboration with Novartis International AG and Professor E Faber (Department of Haemato-Oncology, University Hospital Olomouc, Faculty of Medicine and Dentistry, Palacky University, Czech Republic);
  • Studies of genes and molecular mechanisms involved in regulation of haematopoiesis, their clinical impact and targeted treatment. In collaboration with Professor K Indrak (Department of Haemato-Oncology, University Hospital Olomouc, Faculty of Medicine and Dentistry, Palacky University, Czech Republic);
  • Novel biochemical markers for various types of cancer. In collaboration with Professor B Melichar (Department of Oncology, University Hospital Olomouc, Faculty of Medicine and Dentistry, Palacky University, Czech Republic);
  • Disease mechanism of ADSL patients;
  • Molecular and biochemical cystinuria study. In collaboration with Dr L Kozák, (Faculty Hospital, Brno, Czech Republic) and Dr S Stastna (Institute for Inherited Metabolic Diseases, Charles University in Prague, Czech Republic);
  • The role of cellular transport mechanisms in efficacy of imatinib mesylate treatment in patients with chronic myeloid leukaemia. In collaboration with Dr V Divoky (Department of Biology, Faculty of Medicine and Dentistry, Palacky University, Czech Republic);
  • Diagnosing inherited metabolic disorders by metabolomic approaches. Norwegian University of Science and Technology (Norges teknisk-naturvitenskapelige universitet, NTNU Trondheim, Norway) and SINTEF (Oslo, Norway); and
  • Defects of de novo synthesis of purines – metabolic study. In collaboration with David A Patterson (Department of Biological Sciences, University of Denver, USA).

 

 


Awards


2014-diplom-betkaThe Young Scientists Awards for the best oral presentation in LinStat Conference 2014
Kalivodová A, Hron K, Filzmoser P. Partial least squares discriminant analysis for compositional data: logratio methodology ant its application to metabolomics. Linköping, Sweden, 24. – 28.8. 2014.
Micova-2012-1The price of Czech Society for Clinical Biochemistry for the best publication in year 2012
Mičová K, Friedecký D, Faber E, Adam T. Isotope dilution direct injection mass spectrometry method for determination of four tyrosine kinase inhibitors in human plasma. Talanta. 2012 May 15; 93:307-13
Micova-2012-2The price of Dean of Medical Faculty at Palacky University in Olomouc for the best publication in year 2012
Mičová K, Friedecký D, Faber E, Adam T. Isotope dilution direct injection mass spectrometry method for determination of four tyrosine kinase inhibitors in human plasma. Talanta. 2012 May 15; 93:307-13
Diplom-2012The price of Czech Society for Clinical Biochemistry for the best publication in year 2011
Janečková H, Hron K, Wojtowicz P, Hlídková E, Barešová A, Friedecký D, Zídková L, Hornik P, Behúlová D, Procházková D, Vinohradská H, Pešková K, Bruheim P, Smolka V, Sťastná S, Adam T. Targeted metabolomic analysis of plasma samples for the diagnosis of inherited metabolic disorders. J Chromatogr A. 2012 Feb 24;1226:11-7
The price of Dean of Faculty of Medicine and Dentistry at Palacky University Olomouc for the best publication in the year 2011

Janečková H, Hron K, Wojtowicz P, Hlídková E, Barešová A, Friedecký D, Zídková L, Hornik P, Behúlová D, Procházková D, Vinohradská H, Pešková K, Bruheim P, Smolka V, Sťastná S, Adam T. Targeted metabolomic analysis of plasma samples for the diagnosis of inherited metabolic disorders. J Chromatogr A. 2012 Feb 24;1226:11-7.

The price of Czech Society for Clinical Biochemistry for the best publication in the year 2011
Janečková H, Hron K, Wojtowicz P, Hlídková E, Barešová A, Friedecký D, Zídková L, Hornik P, Behúlová D, Procházková D, Vinohradská H, Pešková K, Bruheim P, Smolka V, Sťastná S, Adam T. Targeted metabolomic analysis of plasma samples for the diagnosis of inherited metabolic disorders. J Chromatogr A. 2012 Feb 24;1226:11-7.

Three awards for the best conference poster presentations in the years 2009, 2010 and 2012 
Janečková H,  Wojtowicz P, Hron K, Brunsvik A, Correa E, Vaughan A, Friedecký D, Bruheim  P, Goodacre R and Adam T; Untargeted metabolomics for diagnosing inborn errors of metabolism. 27. pracovné dni Dedičné metabolické poruchy, Košice, Slovensko, 16.–18. 5. 2012.  Book of Abstract, s. 66. ISBN 978-80-971030-2-6.

Krätschmerová H, Friedecký D., Adam T,  Diagnosis of enzyme defects in purine de novo synthesis by UHPLC-tandem mass spectrometry, 25. pracovné dni Dedičné metabolické poruchy, Trenčianské Teplice, Slovensko 5.–7. 5. 2010 Book of Abstract, p. 17.

Krätschmerová H, Vyskočilová P, Ševčíková J and Adam T Screening test for enzyme defects in the second part of purine de novo synthesis 24. pracovní dny Dědičné metabolické poruchy Hotel Priessnitz, Lázně Jeseník 13.-15. května 2009 Book of Abstract, p. 13.

Student research award of Faculty of Medicine and Dentistry at Palacky University Olomouc in the year 2010 – the 1st place
Krätschmerová H, Friedecký D and Adam T. Diagnosis of enzyme defects in purine de novo synthesis by UHPLC-tandem mass spectrometry. Konference vědeckých prací studentů DSP 2010, Olomouc, 7.–8. 9. 2010. Book of abstract, p. 58, ISBN 978-80-244-2562-7.

The price of Dean of Faculty of Science at Palacky University in Olomouc for the best master thesis in year 2009 – the 3rd placeScreening of inherited metabolic disorders of purine de novo synthesis